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human epidermal melanocytes  (ATCC)


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    ATCC human epidermal melanocytes
    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal <t>melanocytes.</t> (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.
    Human Epidermal Melanocytes, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+normal+melanocytes/Primary+Epidermal+Melanocytes%3B+Normal%2C+Human%2C+Adult/pmc13269842-52-6-13
    Average 99 stars, based on 330 article reviews
    human epidermal melanocytes - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4"

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

    Journal: Human Mutation

    doi: 10.1155/humu/4978880

    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.
    Figure Legend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Techniques Used: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro

    Related Articles

    Expressing:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
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    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Microarray:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Quantitative RT-PCR:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Western Blot:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Comparison:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Control:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Binding Assay:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Methylation:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Software:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Methylated DNA Immunoprecipitation:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Immunofluorescence:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Staining:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Co-Immunoprecipitation Assay:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Negative Control:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Amplification:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Over Expression:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Phospho-proteomics:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    CCK-8 Assay:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Transwell Assay:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Migration:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Flow Cytometry:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.

    Incubation:

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M-254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
    Article Snippet: Human melanoma cell lines (A375, SK-MEL-1 and SK-MEL-28) and human normal melanocytes (PIG1 and HEMn-LP) were purchased from American type culture collection (ATCC, Manassas, VA).. Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).Human melanoma cell lines were incubated in DMEM and human normal melanocytes were incubated in M -254 basal medium (Invitrogen, Carlsbad, CA) supplemented with 1% human melanocyte growth supplement-2 (HMGS-2).

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .All cells were sub-cultured twice a week and kept at 37 °C in a humid environment with 5% CO 2 .

    Article Title: Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico.
    Article Snippet: Cytotoxic effects of A. nilotica flower extract were tested against human hepatocellular carcinoma cells (HepG-2 cells) that were obtained from VACSERA Tissue Culture Unit, Egypt, and human normal melanocytes (HFB-4 cells) that were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).. J. Funct.



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    Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Journal: Human Mutation

    Article Title: A Macrophage/Monocyte‐Related Four‐Gene Signature for Prognostic Assessment of Uveal Melanoma: BTBD6 , C2CD4B , CCL24 , and S100A4

    doi: 10.1155/humu/4978880

    Figure Lengend Snippet: Role of four prognostically relevant genes. (A) Quantitative reverse transcription PCR for the calculated levels of four prognostically relevant genes in human epidermal melanocytes. (B) Validation of the knockdown efficiency of C2CD4B. (C, D) Scratch and Transwell assays exploring the migration and invasion of A375 cells in vitro. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, p > 0.05.

    Article Snippet: Melanoma cell line A375 (CRL‐1619) and human epidermal melanocytes (PCS‐200‐013) were purchased from American Type Culture Collection (ATCC, Manassas, Virginia) and cultured as follows.

    Techniques: Reverse Transcription, Biomarker Discovery, Knockdown, Migration, In Vitro